Difference between revisions of "Part:BBa K1412000"
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This part is consist of Lac promoter and CheZ generator. The expression of CheZ allows <i>E.coli</i> to navigate gradients of natural chemoattractants.  
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This part is consist of Lac promoter and CheZ generator.
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At the same time, we know that Lac promoter is an inverting regulator sensitive to LacI and CAP, while LacI can be inhibited by IPTG. So we can adjust LacI or CAP to make <i>E.coli</i> chemotaxis regularly.
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CheZ can dephosphorylate CheY to make the flagellar motor rotate counterclockwise so that the cell to run and tumble.Without CheZ, CheY is phosphorylated, which can bind to the flagellar motor protein FliM, causing the cell to tumble.so the cell tumbles only.
 +
The expression of CheZ allows <i>E.coli</i> to navigate gradients of natural chemoattractants.  
 +
 
 +
At the same time, we know that Lac promoter is an inverting regulator sensitive to LacI and CAP, while LacI can be inhibited by IPTG. So with LacI protein, CAP protein and IPTG,we can adjust the expression of CheZ via Lac promoter to control the movement of <i>E.coli</i>.
  
 
==='''Usage and Biology'''===
 
==='''Usage and Biology'''===

Revision as of 07:04, 5 September 2014

CheZ generator under Lac promoter


This part is consist of Lac promoter and CheZ generator.

CheZ can dephosphorylate CheY to make the flagellar motor rotate counterclockwise so that the cell to run and tumble.Without CheZ, CheY is phosphorylated, which can bind to the flagellar motor protein FliM, causing the cell to tumble.so the cell tumbles only. The expression of CheZ allows E.coli to navigate gradients of natural chemoattractants.

At the same time, we know that Lac promoter is an inverting regulator sensitive to LacI and CAP, while LacI can be inhibited by IPTG. So with LacI protein, CAP protein and IPTG,we can adjust the expression of CheZ via Lac promoter to control the movement of E.coli.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Experimental Data