Difference between revisions of "Part:BBa K1346011"

 
 
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<partinfo>BBa_K1346011 short</partinfo>
 
<partinfo>BBa_K1346011 short</partinfo>
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Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.
 
Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.
  
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[[File:ASG7-zoomed.png|900px]]
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Compared to the other alpha factor responsive promoters, pASG7 has a high basal expression level, a sharply narrow range of expression, and a mid-level maximum output, comparable to pSAG1's maximum expression rate.
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[[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]]
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[[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]
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Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:26, 3 September 2014

pASG7

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.

ASG7-zoomed.png

Compared to the other alpha factor responsive promoters, pASG7 has a high basal expression level, a sharply narrow range of expression, and a mid-level maximum output, comparable to pSAG1's maximum expression rate.

140722 AFRP GFP DB ROUND 4 zoomed.png


AFRP GFP DB ROUND 4 140722.png

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 252
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 252
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 252
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]