Difference between revisions of "Part:BBa K1346010"

Revision as of 21:24, 3 September 2014

pCLG1

Promoter endogenous to s. cerevisiae that is induced by alpha factor. Characterized on flow cytometer by measuring its mean GFP fluorescence over 7 concentrations of alpha factor.

Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized, meaning pCLG1 is not very responsive to alpha factor.

Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 [[File:CLG1-zoomed.png|900px]]
+Compared to the other alpha factor responsive promoters, pCLG1 has a high basal expression level, a very broad range of expression, and a mid-level maximum output, although the curve suggests that the expression level had not yet been maximized, meaning pCLG1 is not very responsive to alpha factor.
 [[File:140722_AFRP_GFP_DB_ROUND_4_zoomed.png|900px]]
@@ Line 11: / Line 13: @@
 [[File:AFRP_GFP_DB_ROUND_4_140722.png|900px]]
+Alpha factor responsive promoters were selected from the following paper: Roberts, Christopher J., et al (2000 Feb 4). Signaling and Circuitry of Multiple MAPK Pathways Revealed by a Matrix of Global Gene Expression Profiles. Science, 287(5454):873-80. http://www.ncbi.nlm.nih.gov/pubmed/10657304.
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