Difference between revisions of "Part:BBa K1346002"

Latest revision as of 20:43, 3 September 2014

pTEF1(m7) - constitutive promoter

A mutant of the pTEF1 constitutive promoter. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 169

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1346002 short</partinfo>
-One of the mutant pTEF1 constitutive promoters characterized in http://aem.asm.org/content/72/8/5266.long. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.
+A mutant of the pTEF1 constitutive promoter. Seemingly the weakest out of the 5 that the UCSF/UCB team characterized.
-[[File:140630_pTEF1_GFP_ROUND_1.png|500px]]
+[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
+All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in <i>Saccharomyces cerevisiae</i>. Applied and Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
 <!-- Add more about the biology of this part here