Difference between revisions of "Part:BBa K1346001"
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<partinfo>BBa_K1346001 short</partinfo>
 
<partinfo>BBa_K1346001 short</partinfo>
  
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Mutant of pTEF1 with differences in about 8 bp. High functioning compared to pTEF1 and its mutants m3,m7,&m10.
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Mutant of pTEF1 with differences in about 8 bp. High functioning compared to pTEF1 and its mutants m3, m7, & m10.
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* *Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
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* Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
  
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
  
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All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.
  
  

Revision as of 20:41, 3 September 2014

pTEF1(m6) - constitutive promoter

Mutant of pTEF1 with differences in about 8 bp. High functioning compared to pTEF1 and its mutants m3, m7, & m10.

  • Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*

140630 pTEF1 GFP ROUND 1.png

All mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168