Difference between revisions of "Part:BBa K1346000"

Revision as of 20:37, 3 September 2014

pTEF1(m3) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 26 bp. Fairly weak compared to pTEF1 and a few other mutants (m6,m10) , though stronger than m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.

Mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 169

Revision as of 20:12, 3 September 2014 (view source) Jchsueh (Talk \| contribs) ← Older edit		Revision as of 20:37, 3 September 2014 (view source) Jchsueh (Talk \| contribs) Newer edit →
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	[[File:140630 pTEF1 GFP ROUND 1.png\|900px]]		[[File:140630 pTEF1 GFP ROUND 1.png\|900px]]

		+	Mutants selected from: Nevoigt, Elke, et al (2006 Aug). Engineering of Promoter Replacement Cassettes for Fine-Tuning of Gene Expression in Saccharomyces cerevisiae. Applied Environmental Microbiology, 72(8):5266-73. http://www.ncbi.nlm.nih.gov/pubmed/16885275.