Difference between revisions of "Part:BBa K1460003:Design"
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===Source=== | ===Source=== | ||
− | Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori. This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures. | + | Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in <i>H. pylori</i>.<sup>[1]</sup> This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures. |
===References=== | ===References=== | ||
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+ | [1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). <i>Helicobacter pylori</i> nickel-transport gene nixA: Synthesis of catalytically active urease in <i>Escherichia coli</i> independent of growth conditions. <i>Molecular Microbiology</i>, 97-109. |
Latest revision as of 16:01, 26 August 2014
Anderson Promoter + NixA + Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Anderson promoter provides strong constitutive expression of NixA in E. coli.
Source
Anderson promoter is that of part BBa_J23102. Terminator is that of part BBa_B1006. NixA gene is naturally found in H. pylori.[1] This part was synthesized by GenScript in the vector pUC57. Part was then transferred into pSB1C3 using standard cloning procedures.
References
[1] Mobley, H., Garner, R., & Bauerfeind, P. (1995). Helicobacter pylori nickel-transport gene nixA: Synthesis of catalytically active urease in Escherichia coli independent of growth conditions. Molecular Microbiology, 97-109.