Difference between revisions of "Part:BBa K1346003"
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<partinfo>BBa_K1346003 short</partinfo>
 
<partinfo>BBa_K1346003 short</partinfo>
  
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Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
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Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.  
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*Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*
  
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]
 
[[File:140630_pTEF1_GFP_ROUND_1.png|900px]]

Revision as of 21:18, 5 August 2014

pTEF1(m10) - constitutive promoter

Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7.

  • Data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to read GFP levels (arbitrary units)*

140630 pTEF1 GFP ROUND 1.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169