Difference between revisions of "Part:BBa K1346003"
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Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data. | Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data. | ||
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Revision as of 21:17, 5 August 2014
pTEF1(m10) - constitutive promoter
Mutant of pTEF1 promoter with differences in about 4 bp. Very similar promoter activity as pTEF1, weaker than m6, and stronger than m3 & m7. Characterization data derived from placing promoters in front of GFP, inserting into yeast homology, and using Flow Cytometry to gather data.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 169