Difference between revisions of "Part:BBa J119353"

Revision as of 15:28, 7 July 2014

Ammeline Biosensor with Riboswitch N3 + RFP

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N3) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N3 riboswitch is based on the M6C" riboswitch with a strong RBS (AGGAGAA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 742
Illegal AgeI site found at 854
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_J119353 short</partinfo>
-The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N2) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N2 riboswitch is based on the M6C" riboswitch with a weak RBS (CACAGGA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).
+The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (N3) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene. The N3 riboswitch is based on the M6C" riboswitch with a strong RBS (AGGAGAA) and sequestration sequence available for theoretical, complete binding to the RBS when ammeline is not present (see Figure 1A of http://www.pnas.org/content/107/7/2830.full).
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