Difference between revisions of "Part:BBa K1300008"

 
 
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<partinfo>BBa_K1300008 short</partinfo>
 
<partinfo>BBa_K1300008 short</partinfo>
  
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recA + translational GFP to qualitatively test the recA promoter.
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The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions.
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Sequencing results compared with expected DNA sequence
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[[Image:RecA + GFP Picture.png|800px]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:51, 24 June 2014

recA promoter + translational GFP

The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions.


Sequencing results compared with expected DNA sequence RecA + GFP Picture.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865