Difference between revisions of "Part:BBa K1300000"
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Sequencing results compared with expected DNA sequence | Sequencing results compared with expected DNA sequence | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 01:50, 24 June 2014
recA promoter + Key (taRNA)
recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.
Sequencing results compared with expected DNA sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 198