Difference between revisions of "Part:BBa K1300000"
 
(4 intermediate revisions by 2 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K1300000 short</partinfo>
 
<partinfo>BBa_K1300000 short</partinfo>
  
−
recA is a promoter that responds DNA damage that can be caused by UV radiation. The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This part was designed for the purpose of unlocking a cis-repressed ccdB mRNA that is coded for in part BBa_K1300001 as part of a kill switch system.   
+
recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.   
 +
 
 +
 
 +
Sequencing results compared with expected DNA sequence
 +
[[Image:RecA + Trans Picture.png|800px]]
 +
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:50, 24 June 2014

recA promoter + Key (taRNA)

recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.


Sequencing results compared with expected DNA sequence RecA + Trans Picture.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 198