Difference between revisions of "Part:BBa K1300008"
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The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions. | The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions. | ||
| + | |||
| + | Sequencing results compared with expected DNA sequence | ||
| + | [[Image:Recaplusgfp.png|800px]] | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:24, 21 June 2014
recA promoter + translational GFP
The recA promoter is an inducible promoter that responds to DNA damage which can be caused by UV radiation (namely the formation of ssDNA). The purpose of this part is to test the effectiveness of the recA promoter by examining the amount of GFP formed under various UV radiation conditions.
Sequencing results compared with expected DNA sequence
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 865