Difference between revisions of "Part:BBa K1300000"

Revision as of 01:20, 21 June 2014

recA promoter + Key (taRNA)

recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.

Sequencing results compared with expected DNA sequence Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 198

@@ Line 4: / Line 4: @@
 recA is a promoter that responds to DNA damage that can be caused by UV radiation (namely the formation of ssDNA). The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This is achieved by opening up the RBS that the cis-repressive sequence blocked off in the mRNA. As a result, protein expression will be allowed to occur. This part was designed for the purpose of unlocking ccdB crRNA that is coded for in part BBa_K1300001 as part of a kill switch system.
+Sequencing results compared with expected DNA sequence
+[[Image:Recaplustrans.png|800px]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===