Difference between revisions of "Part:BBa K1255003"

Latest revision as of 23:09, 20 June 2014

pConst + Riboswitch + BSMT1

BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.

BSMT1 opt. (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma. Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a RNA thermometer (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 474
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 BSMT1 opt. activated with a constitutive promotor, regulated a riboswitch.
-BSMT1 (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma.  Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a Riboswitch (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.
+BSMT1 opt. (BBa_K1255001) encodes SAM benzoic acid/salicylic acid carboxyl methyltransferase, which is a molecule catalyzes the salicylic acid to methyl salicylate. In order to do it, there must be added 10mM of salicylic acid to produce a winter green aroma.  Besides this device has a constitutive promotor (BBa_J23119), it is regulated by a RNA thermometer (BBa_K115017) that is a RBS but with a loop that unfolds at a temperature around of 32º C.
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