Difference between revisions of "Part:BBa K1300000"

 
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<partinfo>BBa_K1300000 short</partinfo>
 
<partinfo>BBa_K1300000 short</partinfo>
  
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RecA is a UV-light-inducible promoter. Trans is a "key" of sorts, acting on a complementary cis "lock" to allow the translation of ccdB, a kill gene. Together, the RecA+Trans system allows for the kill gene to be translated in the presence of UV light, through the indirect induction by way of the cis-trans system.
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recA is a promoter that responds DNA damage that can be caused by UV radiation. The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This part was designed for the purpose of unlocking a cis-repressed ccdB mRNA that is coded for in part BBa_K1300001 as part of a kill switch system.
  
 
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Revision as of 05:46, 20 June 2014

recA promoter + Key (taRNA)

recA is a promoter that responds DNA damage that can be caused by UV radiation. The trans-activating sequence that recA controls for is a "key" that unlocks cis-repressed RNA (crRNA), which is formed by having a cis-repressive sequence upstream of a gene. This part was designed for the purpose of unlocking a cis-repressed ccdB mRNA that is coded for in part BBa_K1300001 as part of a kill switch system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 198