Difference between revisions of "Part:BBa K1256008"

Latest revision as of 04:04, 10 June 2014

pSB1C3-Plac-SS-DNase

The [http://www.genome.jp/dbget-bin/www_bget?smz:SMD_4069 DNase] coding sequence (cds) of Stenotrophomonas maltophilia D457 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003). The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to two more sites on the gene coding region.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 467
Illegal PstI site found at 674
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 290
Illegal PstI site found at 467
Illegal PstI site found at 674
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1124
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 467
Illegal PstI site found at 674
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 467
Illegal PstI site found at 674
Illegal NgoMIV site found at 410
Illegal NgoMIV site found at 509
Illegal AgeI site found at 434
Illegal AgeI site found at 797
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 935

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1256008 short</partinfo>
-TEST
+[[File: Mingdao_pSB1C3-Plac-SS-DNase.png|400px]]
+The [http://www.genome.jp/dbget-bin/www_bget?smz:SMD_4069 DNase] coding sequence (cds) of Stenotrophomonas maltophilia D457 was obtained from KEGG Genes Database. The gene was cut by NheI and BamHI and ligated to the vector of [https://parts.igem.org/Part:BBa_K1256003 pSB1C3-Plac-SS-NB (Part:BBa_K1256003)]. The cds can be easily exchanged with NheI and BamHI. However, PstI site can no longer be used in this plasmid due to two more sites on the gene coding region.
 <!-- Add more about the biology of this part here