Difference between revisions of "Part:BBa K1256005"

Latest revision as of 02:27, 10 June 2014

pSB1C3-Plac-SS-Magainin-MprF

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of magainin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/m7402?lang=en&region=TW amino acid sequences] of magainin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 362
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2888
Illegal BamHI site found at 2957
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 2339
1000
COMPATIBLE WITH RFC[1000]

Revision as of 02:24, 10 June 2014 (view source) F91445122 (Talk \| contribs) ← Older edit		Latest revision as of 02:27, 10 June 2014 (view source) F91445122 (Talk \| contribs)
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	[[File: Mingdao_pSB1C3-Plac-SS-Magainin-MprF.png\|400px]]		[[File: Mingdao_pSB1C3-Plac-SS-Magainin-MprF.png\|400px]]

−	MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of [https://parts.igem.org/Part:BBa_K1256003 pSB1C3-Plac-SS-NB (Part:BBa_K1256003)] using NheI and BamHI sites. Next, the cds of ~~cecropin~~ was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/~~c6830~~?lang=en&region=TW amino acid sequences] of ~~cecropin~~ was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).	+	MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of [https://parts.igem.org/Part:BBa_K1256003 pSB1C3-Plac-SS-NB (Part:BBa_K1256003)] using NheI and BamHI sites. Next, the cds of magainin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/m7402?lang=en&region=TW amino acid sequences] of magainin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).