Difference between revisions of "Part:BBa K1256005"

Revision as of 02:24, 10 June 2014

pSB1C3-Plac-SS-Magainin-MprF

MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of pSB1C3-Plac-SS-NB (Part:BBa_K1256003) using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/c6830?lang=en&region=TW amino acid sequences] of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 362
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2888
Illegal BamHI site found at 2957
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1283
Illegal NgoMIV site found at 2339
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1256005 short</partinfo>
-TEST
+[[File: Mingdao_pSB1C3-Plac-SS-Magainin-MprF.png|400px]]
+MprF of Bacillus subtilis (DB2)added a RBS (BBa_B0034)was amplified by PCR and cloned into the vector of [https://parts.igem.org/Part:BBa_K1256003 pSB1C3-Plac-SS-NB (Part:BBa_K1256003)] using NheI and BamHI sites. Next, the cds of cecropin was synthesized on the primers and subjeted to Gibson Assembly to make this plasmid. The [http://www.sigmaaldrich.com/catalog/product/sigma/c6830?lang=en&region=TW amino acid sequences] of cecropin was obtained from Sigma-Aldrich, and the nucleotide sequence was optimized for engineering Escherichia coli using the [http://sg.idtdna.com/CodonOpt codon optimization] tool provided by Integrated DNA Technologies (IDT).
 <!-- Add more about the biology of this part here