Difference between revisions of "Part:BBa K1256001:Design"
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[[File:Mingdao PSB1C3-Plac-NB.png|400px]] | [[File:Mingdao PSB1C3-Plac-NB.png|400px]] | ||
| − | NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015) on the vector of pSB1C3. | + | NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015)on the vector of pSB1C3. RFP can easily be exchanged with other coding region and maintain the standard BioBrick assembly rule (E-X-S-P). Furthermore, the front subpart (Plac-RBS) can be cut by EcoRI and NheI site and assembled with other BioBrick parts cut by EcoRI and XbaI. The back subpart (RFP-Tr) can be cut by NheI and PstI and assembled with other BioBrick parts cut by SpeI and PstI. |
===Source=== | ===Source=== | ||
===References=== | ===References=== | ||
Revision as of 14:51, 9 June 2014
pSB1C3-Plac-NB
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found at 1076
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3124
Illegal NheI site found at 221
Illegal SpeI site found at 1077
Illegal PstI site found at 1091
Illegal NotI site found at 1084
Illegal NotI site found at 3130 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 3124
Illegal BamHI site found at 933
Illegal XhoI site found at 2108
Illegal XhoI site found at 3000 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 3124
Illegal suffix found at 1077 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 3124
Plasmid lacks a suffix.
Illegal XbaI site found at 3139
Illegal SpeI site found at 1077
Illegal PstI site found at 1091
Illegal AgeI site found at 781
Illegal AgeI site found at 893 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
NheI and BamHI sites were introduced just behind RBS (B0034) and in front of the terminator (B0015)on the vector of pSB1C3. RFP can easily be exchanged with other coding region and maintain the standard BioBrick assembly rule (E-X-S-P). Furthermore, the front subpart (Plac-RBS) can be cut by EcoRI and NheI site and assembled with other BioBrick parts cut by EcoRI and XbaI. The back subpart (RFP-Tr) can be cut by NheI and PstI and assembled with other BioBrick parts cut by SpeI and PstI.