Difference between revisions of "Part:BBa K624048"

Latest revision as of 14:46, 19 May 2014

Safety Flag

The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.

RBS(trunc.) + LLO + RBS(trunc.) + Inv + RBS(trunc.) + ECFP

Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

All of the ribosome binding sites are from Psmp3 with 6 bps truncated.(BBa_K624013).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1408
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2405
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3022
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
+{{Template:SafetyFlag|reason=[[Safety/Listeriolysin and Invasin | Listeriolysin and Invasin parts]]}}
 <partinfo>BBa_K624048 short</partinfo>
 RBS(trunc.) + LLO + RBS(trunc.)  + Inv + RBS(trunc.) + ECFP
-All of the ribosome binding sites are RBS from Pmsp3 with 6 bps truncated([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
+Listeriolysin O (LLO) is a hemolysin produced by the bacterium Listeria monocytogenes, a pathogen that is responsible for listeriosis. LLO is activated within phagosomes of cells that have phagocytosed L. monocytogenes cells, and lyses the membrane of phagosome. The bacteria is then able to escape into the cytosol without damaging the plasma membrane, and grow intracellularly. This would result in the protection from extracellular immune system.
+Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
+All of the ribosome binding sites are from Psmp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
 <!-- Add more about the biology of this part here