Difference between revisions of "Part:BBa K624062"
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| + | {{Template:SafetyFlag|reason=[[Safety/Listeriolysin and Invasin | Listeriolysin and Invasin parts]]}} | ||
<partinfo>BBa_K624062 short</partinfo> | <partinfo>BBa_K624062 short</partinfo> | ||
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pYMB([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria. | pYMB([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria. | ||
| − | The ribosome binding site is from | + | The ribosome binding site is from Pmsp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]). |
Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization. | Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization. | ||
Latest revision as of 14:42, 19 May 2014
Safety Flag
The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.
Reason: Listeriolysin and Invasin parts
If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.
pYMB essentials + RBS(trunc.) + Inv
pYMB essentials + RBS(trunc.) + Inv
pYMB(BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
The ribosome binding site is from Pmsp3 with 6 bps truncated.(BBa_K624013).
Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1929
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 2546 - 1000COMPATIBLE WITH RFC[1000]
