Difference between revisions of "Part:BBa K624062"

Latest revision as of 14:42, 19 May 2014

Safety Flag

The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.

pYMB essentials + RBS(trunc.) + Inv

pYMB(BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

The ribosome binding site is from Pmsp3 with 6 bps truncated.(BBa_K624013).

Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from Yersinia enterocolitica. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1929
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 2546
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
+{{Template:SafetyFlag|reason=[[Safety/Listeriolysin and Invasin | Listeriolysin and Invasin parts]]}}
 <partinfo>BBa_K624062 short</partinfo>
 pYMB essentials + RBS(trunc.) + Inv
-pYMB is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
+pYMB([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
-The ribosome binding site is from Psmp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
+The ribosome binding site is from Pmsp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
-Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells and induces endocytosis.
+Invasin is an outer membrane protein that allows bacteria to penetrate mammalian cells. It is from ''Yersinia enterocolitica''. Invasin binds to multiple Beta 1 chain integrin receptors on mammalian cells with high affinity, which induces endocytosis and internalization.
 <!-- Add more about the biology of this part here
@@ Line 22: / Line 23: @@
 <partinfo>BBa_K624062 parameters</partinfo>
 <!-- -->
+[[image:pymb_inv.jpg||500px]]