Difference between revisions of "Part:BBa K299810"

Latest revision as of 14:03, 19 May 2014

Safety Flag

The iGEM Safety and Security Committee has placed a Red Flag on this part. This part presents safety risks beyond what is normal for the Registry. Researchers who plan to acquire and use this part should take special care to ensure they use it safely and responsibly. Contact safety [AT] igem [DOT] org with any questions.

Reason: Listeriolysin and Invasin parts

If you are an iGEM team, you must submit a Check-In before acquiring and using this part! See the 2021 Safety Page for more information.

This part is licensed under
Creative BioCommons

B0032+Invasin

Invasin (INV) plays a key role in the initiation of Yersinia enterocolytica and Yersinia pseudotuberculosis infection. Through interaction with a beta1-integrin receptor present on the surface of eucaryotic cell membranes it triggers a signal-transduction pathway leading to internalisation of the whole bacterium in the endocytosis-dependent manner. The strong affinity of invasin to it’s receptor results in highly selective binding to the target molecule. Mammalian cells depleted of beta1-integrin cannot be infected.

Authors:

Cloned by Marta Błaszkiewicz under supervision of Michał Lower.

Construct design

The part consists of B0032 RBS followed by inv gene from Yersinia pestis (horizontal gene transfer form Yersinia pseudotuberculosis). The construct is a fragment of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.

Safety

Bacteria transformed with this part are invasive microorganisms. All safety precautions must be taken when manipulating with transformed strain. It involves obligatory use of laboratory gloves. Work under laminar is strongly advised. All waste should be autoclaved to avoid accidental gene transfer to other bacteria and potential rise of pathogenic organism.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 770
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1387
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
-{{BioCommons}}
-<partinfo>BBa_K299810 short</partinfo>
+{{Template:SafetyFlag|reason=[[Safety/Listeriolysin and Invasin | Listeriolysin and Invasin parts]]}}
+{{BioCommons}}
+<h2><partinfo>BBa_K299810 short</partinfo></h2>
+<p><b>Invasin (INV)</b> plays a key role in the initiation of <i>Yersinia enterocolytica</i> and <i>Yersinia pseudotuberculosis</i> infection. Through interaction with a beta1-integrin receptor present on the surface of eucaryotic cell membranes it triggers a signal-transduction pathway leading to internalisation of the whole bacterium in the endocytosis-dependent manner. The strong affinity of invasin to it’s receptor results in highly selective binding to the target molecule. Mammalian cells depleted of beta1-integrin cannot be infected.</p>
+<p><h3>Authors:</h3>
+Cloned by Marta Błaszkiewicz under supervision of Michał Lower.</p>
+<p><h3>Construct design</h3>
+The part consists of  B0032 RBS followed by inv gene from <i>Yersinia pestis</i> (horizontal gene transfer form <i>Yersinia pseudotuberculosis</i>). The construct is a fragment of the Invasiveness Operon (<html><A HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K299813'>BBa_K299813</A> and <A HREF='https://parts.igem.org/wiki/index.php/Part:BBa_K299815'>BBa_K299815</A></html>) To find out more about it's background and design <html><A HREF='http://2010.igem.org/Team:Warsaw/Stage3'>click here.</A></html></p>
+<p><h3>Safety</h3>
+<b>Bacteria transformed with this part are invasive microorganisms.</b> All safety precautions must be taken when manipulating with transformed strain. It involves obligatory use of laboratory gloves. Work under laminar is strongly advised. All waste should be autoclaved to avoid accidental gene transfer to other bacteria and potential rise of pathogenic organism.</p>