Difference between revisions of "Part:BBa M45117"

 
 
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<partinfo>BBa_M45117 short</partinfo>
 
<partinfo>BBa_M45117 short</partinfo>
  
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This composite part is made out of the uranium inducible (urcA)promoter (BBa_M45114), a ribosome binding site, the GFPuv protein which is a green fluorescent protein (GFP) variant optimized for excitation by UV light, and an extra stop codon to prevent RNA polymerase from reading it through.
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This composite part is a reporter made out of the uranium inducible (urcA) promoter (BBa_M45113), a strong ribosome binding site for E. coli (BBa_B0034), the GFPuv protein (BBa_M45116), and two terminators (BBa_B0010, BBa_B0012) to prevent RNA polymerase from reading the termination step through.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:39, 19 April 2014

Uranium Inducible GFPuv Reporter

This composite part is a reporter made out of the uranium inducible (urcA) promoter (BBa_M45113), a strong ribosome binding site for E. coli (BBa_B0034), the GFPuv protein (BBa_M45116), and two terminators (BBa_B0010, BBa_B0012) to prevent RNA polymerase from reading the termination step through.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1520
    Illegal XhoI site found at 1421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 630
    Illegal SapI.rc site found at 584