Difference between revisions of "Part:BBa M45113:Design"

 
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===Design Notes===
 
===Design Notes===
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The sequence complied with RFC-10 standard assembly, so no design considerations had to be considered during the creation of the sequence as a biobrick.
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The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.
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===Source===
 
===Source===
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This part comes from Caulobacter crescentus CB15 section 318 of 359 of the complete  
 
This part comes from Caulobacter crescentus CB15 section 318 of 359 of the complete  
 
genome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008).
 
genome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008).
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Reference:
 
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Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008.
 
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This reference was retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf
 
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===References===
 
===References===
 +
Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008.Retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf

Latest revision as of 16:08, 17 April 2014


Uranium Inducible (urcA) Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2
    Illegal NgoMIV site found at 317
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 630
    Illegal SapI.rc site found at 584


Design Notes

The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.

Source

This part comes from Caulobacter crescentus CB15 section 318 of 359 of the complete genome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008).

References

Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008.Retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf