Difference between revisions of "Part:BBa M45138:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | ChrR30 and ChrR6 are improved by directed evolution. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082 | ||
+ | Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179 |
Latest revision as of 05:28, 16 April 2014
Type II Secretion of Chromate and Uranium Reductase with TorA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 918
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 685
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ChrR30 and ChrR6 are improved by directed evolution.
Source
References
Barak Y et al. “Analysis of Novel Soluble Chromate and Uranyl Reductases and Generation of an Improved Enzyme by Directed Evolution” Applied and Environmental Microbiology, 2006;72:7074-7082 Barak Y et al. “ Enzyme Improvement in the Absence of Structural Knowledge: a Novel Statistical Approach” International Society for Microbial Ecology, 2008;2:171-179