Difference between revisions of "Part:BBa J119329"

 
 
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<partinfo>BBa_J119329 short</partinfo>
 
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The construct is a biosensor for ammeline.  The P5 promoter drives a riboswitch (M6C) that allows tranlsation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.
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The construct is a biosensor for ammeline.  The P5 promoter drives a riboswitch (M6C'' from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.
  
 
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Latest revision as of 16:57, 3 February 2014

Ammeline Biosensor with Riboswitch M6C'' + RFP

The construct is a biosensor for ammeline. The P5 promoter drives a riboswitch (M6C from http://www.pnas.org/content/early/2010/01/21/0911209107) that allows translation in the presence of ammeline (made by demethylation of melamine) of a RFP reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 740
    Illegal AgeI site found at 852
  • 1000
    COMPATIBLE WITH RFC[1000]