Difference between revisions of "Part:BBa K823027:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K823027 short</partinfo> | <partinfo>BBa_K823027 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | We removed any forbidden restriction sites by site-directed mutagenesis PCR. The | + | We removed any forbidden restriction sites by site-directed mutagenesis PCR. The cloging site also includes a rfp for selection in E.coli. |
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===Source=== | ===Source=== | ||
| − | This part is an igem compatible version of the Bacillus subtilis vector pAX01 | + | This part is an igem compatible version of the ''Bacillus subtilis'' vector pAX01 |
===References=== | ===References=== | ||
| + | |||
| + | [http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl ''et al.''] | ||
Latest revision as of 08:47, 20 December 2013
pSBBs2E: Empty backbone for integration into Bacillus subtilis lacA locus
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NotI site found at 91
Illegal NotI site found at 1622 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix. - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NgoMIV site found at 2591
Illegal NgoMIV site found at 4919 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 4743
Illegal SapI site found at 3660
Design Notes
We removed any forbidden restriction sites by site-directed mutagenesis PCR. The cloging site also includes a rfp for selection in E.coli.
Source
This part is an igem compatible version of the Bacillus subtilis vector pAX01
References
[http://www.ncbi.nlm.nih.gov/pubmed/11274134 Härtl et al.]