Difference between revisions of "Part:BBa K1092023"
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<partinfo>BBa_K1092023 short</partinfo> | <partinfo>BBa_K1092023 short</partinfo> | ||
| − | T7-RBS-ssDsbA-PDZ | + | This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Domain-Voltage sensor peptide-RFP N terminus (BBa_K1092024), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world. |
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Latest revision as of 11:50, 15 November 2013
T7-RBS-ssDsbA-PDZ Ligand-Voltage Switch-RFP C terminus
This is biobrick is the counterpart of the other biobrick T7-RBS-ssDsbA-PDZ Domain-Voltage sensor peptide-RFP N terminus (BBa_K1092024), and is composed of the voltage switch (BBa_K1092009 & BBa_K1092010) and the RFP fragments (BBa_K1092105 & BBa_K1092106). In this Example, we demonstrate the possibility of using the voltage switch as a fast control of fluorescent signal. We made use of the biomolecular fluorescent complementation to achieve this purpose, and we worked on making the first reversible BiFC reaction in the world.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 405
Illegal AgeI site found at 517 - 1000COMPATIBLE WITH RFC[1000]