Difference between revisions of "Part:BBa K1131006:Experience"
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===Applications of BBa_K1131006=== | ===Applications of BBa_K1131006=== | ||
| + | [[File:OleD-K-UDPG-bleck-short.png|500px]]<br> | ||
| + | Absorbance data obtained from HPLC at 270nm. Reaction containing Kaempferol, OleD, and donor molecule UDP-glucose. Peaks for kaempferol and kaempferol glucoside are observed demonstrating the successful purification of active OleD. | ||
| + | [[File:HPLC_Indican_Black_1.png|500px]]<br> | ||
| + | Absorbance data obtained from HPLC at 270nm. Reaction containing indoxyl, OleD, and donor molecule UDP-glucose peaks for indican and sodium complexed indican are observed along with the precursor indoxyl.<br> | ||
| + | [[File:T4_IPO4_UDPG_Black_1.png|500px]]<br> | ||
| + | Mass spectrometry data for the elution fraction at a retention time of 9.51 (Indican peak in HPLC data shown in previous figure). Peaks matching the mass of the precursor indoxyl (134), indican (296), and sodium complexed indican (318) were found. All unlabeled peaks did not correspond to expected ions.<br> | ||
| + | <!-- Uncomment this to enable Functional Parameter display | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 05:16, 2 November 2013
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Applications of BBa_K1131006
Absorbance data obtained from HPLC at 270nm. Reaction containing Kaempferol, OleD, and donor molecule UDP-glucose. Peaks for kaempferol and kaempferol glucoside are observed demonstrating the successful purification of active OleD.
Absorbance data obtained from HPLC at 270nm. Reaction containing indoxyl, OleD, and donor molecule UDP-glucose peaks for indican and sodium complexed indican are observed along with the precursor indoxyl.
Mass spectrometry data for the elution fraction at a retention time of 9.51 (Indican peak in HPLC data shown in previous figure). Peaks matching the mass of the precursor indoxyl (134), indican (296), and sodium complexed indican (318) were found. All unlabeled peaks did not correspond to expected ions.
UNIQ21b3d469a7abda03-partinfo-00000000-QINU
UNIQ21b3d469a7abda03-partinfo-00000001-QINU