Difference between revisions of "Part:BBa K1131006"

Revision as of 06:04, 30 October 2013

oleD glucosyltransferase; S. antibiotics (characterized)

This oleandomycin glucosyltransferase was found to glucosylate indoxyl to produce indoxyl-B-glucoside, or indican. To the best of our knoweldge, this is the first recombinant enzyme used to produce indican. Kaempferol is another substrate of this enzyme. It is known to highly promiscuous in substrate specificity and glucosylates a variety of phenolics and anthrocyanins. The sequence shown here corresponds to that of UniProt ID Q3HTL6, and encodes the ORF only. This is the characterized and slightly altered version of BBa1131004. The last 15 amino acids have been removed from this version increasing its solubility greatly. A mutated version known to have even broader activity was added to registry as well.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 688
Illegal NgoMIV site found at 928
Illegal NgoMIV site found at 1118
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 387
Illegal BsaI.rc site found at 653
Illegal BsaI.rc site found at 1078

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 <partinfo>BBa_K1131006 short</partinfo>
-This oleandomycin glucosyltransferase was found to glucosylate indoxyl to produce indoxyl-B-glucoside, or indican. To the best of our knoweldge, this is the first recombinant enzyme used to produce indican. Kaempferol is another substrate of this enzyme. It is known to highly promiscuous in substrate specificity and glucosylates a variety of phenolics and anthrocyanins. The sequence shown here corresponds to that of UniProt ID Q3HTL6, and encodes the ORF only. This is the characterized and slightly altered version of BBa_K1131004. The last 15 amino acids have been removed from this version increasing its solubility greatly.
+This oleandomycin glucosyltransferase was found to glucosylate indoxyl to produce indoxyl-B-glucoside, or indican. To the best of our knoweldge, this is the first recombinant enzyme used to produce indican. Kaempferol is another substrate of this enzyme. It is known to highly promiscuous in substrate specificity and glucosylates a variety of phenolics and anthrocyanins. The sequence shown here corresponds to that of UniProt ID Q3HTL6, and encodes the ORF only. This is the characterized and slightly altered version of [https://parts.igem.org/Part:BBa_K1131004 BBa1131004]. The last 15 amino acids have been removed from this version increasing its solubility greatly. A mutated version known to have even broader activity was [https://parts.igem.org/Part:BBa_K1131007 added to registry] as well.
 <!-- Add more about the biology of this part here