Difference between revisions of "Part:BBa K1104101"

Latest revision as of 19:47, 29 October 2013

RFP-FimH(mannose-binding domain) fusion protein

　　This biobrick is cloned from the 22nd to the 179th amino acid ,which is the mannose-binding domain in the FimH protein. FimH protein, a protein expressed in Escherichia coli K-12 substrain MG1655, has a mannose-binding domain from the 22nd to 179th amino acid. FimH protein is originally located on the tip of the fimbria of the E. coli, which will adhere to the mannose on the host's cell membrane, allowing the bacteria to colonize various host tissues. In the project of 2013 NYMU_Taipei team, they have used this protein to block the invasion of a pathogen called Nosema ceranae by binding to the mannose polymer on the polar tube.

Usage and Biology

　　Since mannose polymer is often seen in pathogens, and its function is often related to invade the host cell. This biobrick can serve as a weapon to stop these pathogen's infection. Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 555
Illegal AgeI site found at 667
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1104101 short</partinfo>
-FimH protein, a protein expressed in Escherichia coli K-12 substr MG1655, has a mannose-binding site from the 22nd to 179th amino acid. FimH protein is originally located on the tip of the fimbria of the E. coli, which will adhere to the mannose on the host's cell membrane, allowing the bacteria to colonize various host tissues. In our project we managed to use it to bind the mannose polymer, which serves as a sticky end to bind the host cell, on top of the PTP1, in order to stop the adhesion between the polar filament and the epithelial cell of the bee's midgut.
+　　This biobrick is cloned from the 22nd to the 179th amino acid ,which is the mannose-binding domain in the FimH protein. FimH protein, a protein expressed in Escherichia coli K-12 substrain MG1655, has a mannose-binding domain from the 22nd to 179th amino acid. FimH protein is originally located on the tip of the fimbria of the E. coli, which will adhere to the mannose on the host's cell membrane, allowing the bacteria to colonize various host tissues. In the project of 2013 NYMU_Taipei team, they have used this protein to block the invasion of a pathogen called ''Nosema ceranae'' by binding to the mannose polymer on the polar tube.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+　　Since mannose polymer is often seen in pathogens, and its function is often related to invade the host cell. This biobrick can serve as a weapon to stop these pathogen's infection.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>