Difference between revisions of "Part:BBa K1021020"
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Revision as of 03:49, 29 October 2013
PT7+GFP
The gene for green fluorescent protein sits downstream of the T7 constitutive promoter. This gene can be naturally expressed in Escherichia coli BL21-A1 in conjunction with T7 polymerase and can be expressed in Escherichia coli DH5α after co-transformation.
Usage and Biology
- The part was constructed from BBa_I712074 and BBa_E0040.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 696