Difference between revisions of "Part:BBa K1021032"
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The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi. | The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi. | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | *The part was constructed from BBa_I712074 and BBa_K1021001. | ||
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Latest revision as of 03:47, 29 October 2013
PT7+nptII
The neomycin phosphotransferase gene (nptII), which also confers resistance to geneticin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.
Usage and Biology
- The part was constructed from BBa_I712074 and BBa_K1021001.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 848
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 681
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 530
Illegal SapI.rc site found at 740