Difference between revisions of "Part:BBa K1021009"
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<partinfo>BBa_K1021009 short</partinfo> | <partinfo>BBa_K1021009 short</partinfo> | ||
− | PpelA is an inducible pectate lyase promoter from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled gene expression can be achieved by cloning the desired gene downstream of PpelA. | + | PpelA is an inducible pectate lyase promoter from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled gene expression can be achieved by cloning the desired gene downstream of PpelA. PpelA was constructed by digesting the part pHNU3pelA with the BamHI and KpnI restriction enzymes and ligating the digested fragment into the pSB1C3 backbone appended with BamHI and KpnI restriction sites. |
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Revision as of 02:47, 29 October 2013
PpelA
PpelA is an inducible pectate lyase promoter from Aspergillus nidulans. It is repressed by glucose and inducible by polygalacturonic acid. Thus, controlled gene expression can be achieved by cloning the desired gene downstream of PpelA. PpelA was constructed by digesting the part pHNU3pelA with the BamHI and KpnI restriction enzymes and ligating the digested fragment into the pSB1C3 backbone appended with BamHI and KpnI restriction sites.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]