Difference between revisions of "Part:BBa K1189032:Design"
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<partinfo>BBa_K1189032 short</partinfo> | <partinfo>BBa_K1189032 short</partinfo> | ||
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===Source=== | ===Source=== | ||
| − | + | This part was synthesized IDT G-blocks and is in the process of being assembled together. | |
===References=== | ===References=== | ||
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| + | <li>Heckman, K. L., & Pease, L. R. (2007). Gene splicing and mutagenesis by PCR-driven overlap extension. Nature protocols, 2(4), 924-932. | ||
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| + | <li>Meckler, J.F., Bhakta, M.S., Kim, M.S., Ovadia, R., Habrian, C.H., Zykovich, A., ... Baldwin, E.P. (2013). Quantitative analysis of TALE-DNA interactions suggests polarity effects. <i>Nucleic Acids Research</i>, 41(7), 4118–28. doi:10.1093/nar/gkt085 | ||
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| + | <li>Perna, N. T., Plunkett, G., Burland, V., Mau, B., Glasner, J. D., Rose, D. J., ... Blattner, F. R. (2000) Genome sequence of enterohaemorrhagic <i>Escherichia coli</i> O157:H7. <i>Letters to Nature</i>. 409, 529-533. doi:10.1038/35054089 | ||
Latest revision as of 01:20, 29 October 2013
EHEC TALE1 (binds to 19bp of stx2 gene)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2404
Illegal BamHI site found at 2539 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A KasI restriction cut site is incorporated at the end of the part so that we can swap the TALEs we currently have in our parts with this one.
Source
This part was synthesized IDT G-blocks and is in the process of being assembled together.