Difference between revisions of "Part:BBa K1021033"

 
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<partinfo>BBa_K1021033 short</partinfo>
 
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The hygromycin phosphotransferase gene (hph) sits downstream of the T7 promoter; this construct can be used as a selectable marker in T7-based expression studies in fungi.
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The hygromycin phosphotransferase gene (hph), which also confers resistance to hygromycin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.  
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 00:28, 29 October 2013

PT7+hph

The hygromycin phosphotransferase gene (hph), which also confers resistance to hygromycin, sits downstream of the T7 promoter; this construct can be used as a selectable marker in co-transformation with T7 polymerase in fungi.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 391
  • 1000
    COMPATIBLE WITH RFC[1000]