Difference between revisions of "Part:BBa K1139200:Design"

 
 
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<partinfo>BBa_K1139200 short</partinfo>
 
<partinfo>BBa_K1139200 short</partinfo>
  
 
<partinfo>BBa_K1139200 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1139200 SequenceAndFeatures</partinfo>
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===Design Notes===
 
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No
 
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===Source===
 
===Source===
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<i>phoA</i> promoter region of <i>E. coli</i> was amplified from MG1655 genomic DNA by PCR.<br>
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The sequence is amplified from E. coli(MG1655) by PCR.
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Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)<br>
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Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)<br>  [http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp <i>details</i>]
  
 
===References===
 
===References===
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1. M. Dollard and P. Billard. (2003) Whole-cell bacterial sensors for the monitoring of phosphate bioavailability. Journal of Microbiological Methods, 55, 221– 229<br>
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2. H. Shinagawa, K. Makino and A. Nakata. (1983) Regulation of the pho Regulon in Escherichia coli K-12. Genetic and Physiological Regulation of the Positive Regulatory Gene phoB. J. Mol. Biol, 168, 477-488<br>
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3. Y. Hsieh and B. L. Wanner. (2010) Global regulation by the seven-component Pi signaling system. Current Opinion in Microbiology, 13, 198-203<br>
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4. F. C. Neidhardt, P. L. Bloch and D. F. Smith. (1974) Culture Medium for Enterobacteria. Journal of Bacteriology, 119(3), 736-47<br>
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5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)<br>
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6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32<br>

Latest revision as of 21:13, 28 October 2013

phoA promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

phoA promoter region of E. coli was amplified from MG1655 genomic DNA by PCR.
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)
[http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp details]

References

1. M. Dollard and P. Billard. (2003) Whole-cell bacterial sensors for the monitoring of phosphate bioavailability. Journal of Microbiological Methods, 55, 221– 229
2. H. Shinagawa, K. Makino and A. Nakata. (1983) Regulation of the pho Regulon in Escherichia coli K-12. Genetic and Physiological Regulation of the Positive Regulatory Gene phoB. J. Mol. Biol, 168, 477-488
3. Y. Hsieh and B. L. Wanner. (2010) Global regulation by the seven-component Pi signaling system. Current Opinion in Microbiology, 13, 198-203
4. F. C. Neidhardt, P. L. Bloch and D. F. Smith. (1974) Culture Medium for Enterobacteria. Journal of Bacteriology, 119(3), 736-47
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32