Difference between revisions of "Part:BBa K737024:Experience"

Latest revision as of 21:11, 28 October 2013

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Applications of BBa_K737024

User Reviews

UNIQbdae2495b7e3ebda-partinfo-00000000-QINU UNIQbdae2495b7e3ebda-partinfo-00000001-QINU

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Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139201)

Fig. 1. Our induction assay result for our phoA promoter

Fig. 2. Our induction assay result for OUC-China 2012's phoB promoter

Compared to OUC-China’s phosphate sensor part including the phoB promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).

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 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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 <b>1. Sequence</b><br>
 No NotI restriction site in prefix and suffix. <br>
-bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)<br>
+bp deletion and 2bp mutation exist between the promoter and RBS.<br>
 <b>2. Result of our experiment</b><br>
-We improved this OUC-China’s phosphate sensor part including <i>phoB</i> promoter by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139200</partinfo>)<br>
+We improved this OUC-China’s phosphate sensor part including the <i>phoB</i> promoter, by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139201</partinfo>)<br>
-[[Image:Titech2013_parts_K1139201_Fig4.jpg|thumb|left|500px|'''Fig. 1.''' Our <i>phoA</i> promoter assay result]]
+[[Image:Titech2013_part_K1139201_Fig4.jpg|thumb|left|250px|'''Fig. 1.''' Our induction assay result for our <i>phoA</i> promoter]]
-[[Image:Titech2013_parts_K1139201_Fig5.jpg|thumb|left|500px|'''Fig. 2.''' OUC-China 2012’s <i>phoB</i> promoter assay result (converted to bar chart)]]
+[[Image:Titech2013_part_K1139201_Fig5.jpg|thumb|left|250px|'''Fig. 2.''' Our induction assay result for OUC-China 2012's <i>phoB</i> promoter]]
 <br><br><br><br><br><br><br><br><br><br><br><br>
-Compared to OUC-China’s phosphate sensor part including <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).
+Compared to OUC-China’s phosphate sensor part including the <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).