Difference between revisions of "Part:BBa K737024:Experience"

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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_K266000 AddReview 1</partinfo>
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<partinfo>BBa_K266000 AddReview 5</partinfo>
 
<I>Tokyo-Tech iGEM 2013</I>
 
<I>Tokyo-Tech iGEM 2013</I>
 
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<b>1. Sequence</b><br>
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No NotI restriction site in prefix and suffix. <br>
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1bp deletion and 2bp mutation exist between the promoter and RBS.<br>
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<b>2. Result of our experiment</b><br>
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We improved this OUC-China’s phosphate sensor part including the <i>phoB</i> promoter, by amplifying the <i>phoA</i> promoter region of <i>E. coli</i> (MG1655) and ligating it upstream of GFP part (<partinfo>BBa_K1139201</partinfo>)<br>
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[[Image:Titech2013_part_K1139201_Fig4.jpg|thumb|left|250px|'''Fig. 1.''' Our induction assay result for our <i>phoA</i> promoter]]
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[[Image:Titech2013_part_K1139201_Fig5.jpg|thumb|left|250px|'''Fig. 2.''' Our induction assay result for OUC-China 2012's <i>phoB</i> promoter]]
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<br><br><br><br><br><br><br><br><br><br><br><br>
  
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No NotI restriction site in prefix and suffix.
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Compared to OUC-China’s phosphate sensor part including the <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).
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1bp deletion and 2bp mutation exist between promoter and RBS. (They are not fatal to the function)
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Latest revision as of 21:11, 28 October 2013

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K737024

User Reviews

UNIQf7cc86982404837a-partinfo-00000000-QINU UNIQf7cc86982404837a-partinfo-00000001-QINU

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Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139201)

Fig. 1. Our induction assay result for our phoA promoter
Fig. 2. Our induction assay result for OUC-China 2012's phoB promoter













Compared to OUC-China’s phosphate sensor part including the phoB promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).