Difference between revisions of "Part:BBa K1021005"
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− | The Cre recombinase can be used for site-specific recombination of constructs flanked by lox sites (BBa_J61046). | + | The Cre recombinase can be used for site-specific recombination of constructs flanked by lox sites (BBa_J61046). The Cre-Lox recombination system can be used for gene manipulation as as a biosafety mechanism. As a biosafety mechanism, its action is to remove engineered genes flanked by lox sequences. |
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Revision as of 19:31, 28 October 2013
Cre recombinase
The Cre recombinase can be used for site-specific recombination of constructs flanked by lox sites (BBa_J61046). The Cre-Lox recombination system can be used for gene manipulation as as a biosafety mechanism. As a biosafety mechanism, its action is to remove engineered genes flanked by lox sequences.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 356
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 42
- 1000COMPATIBLE WITH RFC[1000]