Difference between revisions of "Part:BBa K1088010"

Latest revision as of 18:48, 28 October 2013

E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib

This part consist of the dxs gene derived from E. coli fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the lac promoter and has a strong RBS. To repress expression from the lac promoter, the lacI:LVA (gene under a constitutive promoter with a strong RBS and a efficient terminator) was placed upstream to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3937
Illegal SapI.rc site found at 2127

@@ Line 3: / Line 3: @@
 This part consist of the ''dxs'' gene derived from ''E. coli'' fused to GFP at the translational level with a 10 AA linker between the proteins. The reporter fusion is under the control of the ''lac'' promoter and has a strong RBS.
-To repress expression from the ''lac'' promoter, the lacI:LVA (gene under a constitutive promoter with a strong RBS and a efficient terminator) was placed counter-clockwise to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
+To repress expression from the ''lac'' promoter, the ''lacI:LVA'' (gene under a constitutive promoter with a strong RBS and a efficient terminator) was placed upstream to the reporter fusion. The repression can be relieved with addition of IPTG, which binds and inhibits the function of LacI:LVA.
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