Difference between revisions of "Part:BBa K1088012"
 
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<partinfo>BBa_K1088012 short</partinfo>
 
<partinfo>BBa_K1088012 short</partinfo>
  
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This part the ''dxs'' derived from ''E.coli'' (BBa_K1088000) controlled by the common lac promoter. Notice that overexpression of LacI is required for regulation of the Lac promoter on high copy plasmids.
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This part contains the ''dxs'' gene derived from ''E. coli'' ([https://parts.igem.org/Part:BBa_K118000 BBa_K118000]) controlled by the ''lac'' promoter and has a strong RBS. The part was build to increase the flow through the MEP (methylerythritol phosphate) pathway, and to test whether Dxs derived from ''E. coli'' or ''B. subtilis'' increased the flow through the MEP pathway the most in ''E. coli''.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 18:24, 28 October 2013

E. coli dxs (lac promoter without lac inhibitor)

This part contains the dxs gene derived from E. coli (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP (methylerythritol phosphate) pathway, and to test whether Dxs derived from E. coli or B. subtilis increased the flow through the MEP pathway the most in E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 946