Difference between revisions of "Part:BBa K1031018"

 
 
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<partinfo>BBa_K1031018 short</partinfo>
 
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a>
  
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Psal-&#966;R73&#916;
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<img src="https://static.igem.org/mediawiki/igem.org/c/c9/Peking_Logo.jpg" style="width:960px;"/>
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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== '''Characteristics''' ==
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K1031018 is composed of ''Psal'' promoter activated by NahR when exposed to salicylic acid, RBS <html><a href="https://parts.igem.org/Part:BBa_B0034">B0034</a></html>, RiboJ and φR73δ, which activates hybrid promoter thus sfGFP is expressed. This is a fundamental component of Band-pass Filter which needs further combination with a biosensor NahR and an inhibition circuit.
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<img src="https://static.igem.org/mediawiki/2013/thumb/8/82/Peking2013_part_Psal-phiR73.png/800px-Peking2013_part_Psal-phiR73.png"
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style="width:800px; margin-left:90px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of <i>Psal</i>-φR73δ and its' function to activate the hybrid promoter. This part is shown in the dashed box in blue.
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== '''Sequence and Features''' ==
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1031018 SequenceAndFeatures</partinfo>
 
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Latest revision as of 18:20, 28 October 2013

Psal-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Characteristics

K1031018 is composed of Psal promoter activated by NahR when exposed to salicylic acid, RBS B0034, RiboJ and φR73δ, which activates hybrid promoter thus sfGFP is expressed. This is a fundamental component of Band-pass Filter which needs further combination with a biosensor NahR and an inhibition circuit.

Fig 1 Construction of Psal-φR73δ and its' function to activate the hybrid promoter. This part is shown in the dashed box in blue.


Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]