Difference between revisions of "Part:BBa K1031019"
 
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<partinfo>BBa_K1031017 short</partinfo>
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<partinfo>BBa_K1031019 short</partinfo>
 
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<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a>
 
<p>For detailed information concerning Band-pass Filter, please visit <a href="http://2013.igem.org/Team:Peking/Project/BandpassFilter">2013 Peking iGEM Band-pass Filter</a>
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== '''Characteristics''' ==
 
== '''Characteristics''' ==
  
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K1031019 is designed to test the activation effect of φR73Δ to hybrid promoter. It is composed of NahR, Psal promoter which is activated by NahR when exposed to its' inducer salicylic acid, RBS <html><a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>, RiboJ and φR73Δ.
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K1031019 is designed to test the activation effect of φR73δ to hybrid promoter. It is composed of NahR, ''Psal'' promoter which is activated by NahR when exposed to its' inducer salicylic acid, RBS <html><a href="https://parts.igem.org/Part:BBa_B0034">B0034</a>, RiboJ and φR73δ.
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<img src="https://static.igem.org/mediawiki/2013/thumb/b/b3/Peking2013_part_K1031019.png/800px-Peking2013_part_K1031019.png"
 
<img src="https://static.igem.org/mediawiki/2013/thumb/b/b3/Peking2013_part_K1031019.png/800px-Peking2013_part_K1031019.png"
 
style="width:800px; margin-left:90px" />
 
style="width:800px; margin-left:90px" />
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<p style="text-align:center"><b>Fig 1</b> Construction of activation circuit of Band-pass Filter. The combination of φR73Δ with hybrid promoter and sfGFP could test if φR73Δ really works as an activator.  
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<p style="text-align:center"><b>Fig 1</b> Construction of activation circuit of Band-pass Filter. The combination of φR73δ with hybrid promoter and sfGFP could test if φR73δ really works as an activator.  
 
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== '''Data shown''' ==
 
== '''Data shown''' ==
  
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Previous work done by Dr. Lou Chunbo has proved the activation effect of φR73Δ to hybrid promoter. This activation circuit is exposed to the typical inducer of NahR, salicylic acid, and the expression level of φR73Δ is controlled by tuning the inducer's concentration. The activation effect of φR73Δ to hybrid promoter is shown via fluorescence intensity.
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We had proved the activation effect of φR73δ to hybrid promoter. This activation circuit is exposed to the typical inducer of NahR, 4-MeSaA, and the expression level of φR73δ is controlled by changing the inducer's concentration. The activation effect of φR73δ to hybrid promoter is shown via induction ratio calculated from fluorescence intensity.
  
 
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<img src="https://static.igem.org/mediawiki/igem.org/thumb/8/84/Peking2013_part_PhiR_73.jpg/800px-Peking2013_part_PhiR_73.jpg"
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<img src="https://static.igem.org/mediawiki/2013/thumb/0/00/Peking2013_part_NahR-psal-phiR73.jpg/772px-Peking2013_part_NahR-psal-phiR73.jpg"
 
style="width:600px;margin-left:170px" />
 
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<p style="text-align:center"><b>Fig 2</b> Effect of activation circuit of Band-pass Filter. Horizontal axis stands for different concentration of NahR inducer, salicylic acid. Vertical axis represents fluorescence intensity. From Fig 2 it is obvious that φR74Δ indeed activates the hybrid promoter, thus initiates the expression of sfGFP. (Data from Dr. Lou Chunbo ????)
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<p style="text-align:center"><b>Fig 2</b> Effect of activation circuit of Band-pass Filter. Horizontal axis stands for different concentrations of NahR inducer 4-MeSaA. Vertical axis represents induction ratio. From <b>Fig 2</b> it is obvious that φR74δ indeed activates the hybrid promoter, thus initiates the expression of sfGFP.
 
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Latest revision as of 18:03, 28 October 2013

NahR-Psal-B0034-RiboJ-φR73δ

For detailed information concerning Band-pass Filter, please visit 2013 Peking iGEM Band-pass Filter


Characteristics

K1031019 is designed to test the activation effect of φR73δ to hybrid promoter. It is composed of NahR, Psal promoter which is activated by NahR when exposed to its' inducer salicylic acid, RBS B0034, RiboJ and φR73δ.

Fig 1 Construction of activation circuit of Band-pass Filter. The combination of φR73δ with hybrid promoter and sfGFP could test if φR73δ really works as an activator.



Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
  • 1000
    COMPATIBLE WITH RFC[1000]



Data shown

We had proved the activation effect of φR73δ to hybrid promoter. This activation circuit is exposed to the typical inducer of NahR, 4-MeSaA, and the expression level of φR73δ is controlled by changing the inducer's concentration. The activation effect of φR73δ to hybrid promoter is shown via induction ratio calculated from fluorescence intensity.

Fig 2 Effect of activation circuit of Band-pass Filter. Horizontal axis stands for different concentrations of NahR inducer 4-MeSaA. Vertical axis represents induction ratio. From Fig 2 it is obvious that φR74δ indeed activates the hybrid promoter, thus initiates the expression of sfGFP.