Difference between revisions of "Part:BBa K737024:Experience"

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Compared to OUC-China’s phosphate sensor part including <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).
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Compared to OUC-China’s phosphate sensor part including the <i>phoB</i> promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (<partinfo>BBa_K1139200</partinfo>, <partinfo>BBa_K1139201</partinfo>).

Revision as of 16:06, 28 October 2013


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K737024

User Reviews

UNIQf3bad667dc07fb75-partinfo-00000000-QINU UNIQf3bad667dc07fb75-partinfo-00000001-QINU

•••••

Tokyo-Tech iGEM 2013

1. Sequence
No NotI restriction site in prefix and suffix.
1bp deletion and 2bp mutation exist between the promoter and RBS.

2. Result of our experiment
We improved this OUC-China’s phosphate sensor part including the phoB promoter, by amplifying the phoA promoter region of E. coli (MG1655) and ligating it upstream of GFP part (BBa_K1139200)

Fig. 1. Our phoA promoter assay result
Fig. 2. OUC-China 2012’s phoB promoter assay result (converted to bar chart)













Compared to OUC-China’s phosphate sensor part including the phoB promoter (Fig. 2), our phosphate sensor part shows a clearer result (Fig. 1) (BBa_K1139200, BBa_K1139201).