Difference between revisions of "Part:BBa K1139201:Design"

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===Source===
 
===Source===
  
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<i>phoA</i> promoter is extracted from <i>E. coli</i> (K-12) genome by PCR. [http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp <i>more information</i>]
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<i>phoA</i> promoter region of <i>E. coli</i> was amplified from MG1655 genomic DNA by PCR.<br>
 +
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)<br>
 +
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)<br>  [http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp <i>details</i>]
  
 
===References===
 
===References===
−
1. M. Dollard et al. “Whole-cell bacterial sensors for the monitoring of phosphate availability” Journal of Microbiological Methods (2003)<br>
+
1. M. Dollard and P. Billard. (2003) Whole-cell bacterial sensors for the monitoring of phosphate bioavailability. Journal of Microbiological Methods, 55, 221– 229<br>
−
2. H. Shinagawa et al. “Regulation of the pho Regulon in Escherichia coli K-12” J. Mol. Biol. (1983)<br>
+
2. H. Shinagawa, K. Makino and A. Nakata. (1983) Regulation of the pho Regulon in Escherichia coli K-12. Genetic and Physiological Regulation of the Positive Regulatory Gene phoB. J. Mol. Biol, 168, 477-488<br>
−
3. Y. Hsieh et al. “Global regulation by the seven-component Pi signaling system” Current Opinion in Microbiology (2010)<br>
+
3. Y. Hsieh and B. L. Wanner. (2010) Global regulation by the seven-component Pi signaling system. Current Opinion in Microbiology, 13, 198-203<br>
−
4. F. Neidhardt et al. “Culture Medium for Enterobacteria” Journal of Bacteriology (1974)<br>
+
4. F. C. Neidhardt, P. L. Bloch and D. F. Smith. (1974) Culture Medium for Enterobacteria. Journal of Bacteriology, 119(3), 736-47<br>
 
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)<br>
 
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)<br>
 
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32<br>
 
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32<br>

Latest revision as of 15:46, 28 October 2013

PphoA-GFP-TT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 754


Design Notes

sequence confirmed

Source

phoA promoter region of E. coli was amplified from MG1655 genomic DNA by PCR.
Upstream primer (5’-acgtgaattcgcggccgcttctagagaaagttaatcttttcaacagctgtcataaag-3’)
Downstream primer (5’-ccgctactagtaaatacattaaaaaataaaaacaaagcgactataagtctc-3’)
[http://regulondb.ccg.unam.mx/operon?term=ECK120014865&format=jsp&organism=ECK12&type=operon#phoAp details]

References

1. M. Dollard and P. Billard. (2003) Whole-cell bacterial sensors for the monitoring of phosphate bioavailability. Journal of Microbiological Methods, 55, 221– 229
2. H. Shinagawa, K. Makino and A. Nakata. (1983) Regulation of the pho Regulon in Escherichia coli K-12. Genetic and Physiological Regulation of the Positive Regulatory Gene phoB. J. Mol. Biol, 168, 477-488
3. Y. Hsieh and B. L. Wanner. (2010) Global regulation by the seven-component Pi signaling system. Current Opinion in Microbiology, 13, 198-203
4. F. C. Neidhardt, P. L. Bloch and D. F. Smith. (1974) Culture Medium for Enterobacteria. Journal of Bacteriology, 119(3), 736-47
5. CCG/UNAM. Regulon DB. http://regulondb.ccg.unam.mx/index.jsp (accessed 2013-09-25)
6. D. R. Hoagland and D. I. Arnon (1950), The water-culture method for growing plants without soil. California agrigultual experiment station circular, 347, 1-32