Difference between revisions of "Part:BBa K1129012"
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This part is computationally verified to target all of 13a, K30, T3 and T7 genomes | This part is computationally verified to target all of 13a, K30, T3 and T7 genomes | ||
| − | Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner | + | Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features |
| − | http://en.wikipedia.org/wiki/CRISPR | + | |
Revision as of 00:10, 28 October 2013
T7 Repeat/Spacer Region of T7 Bacteriophage
T7 Repeat/Spacer Region of Streptococcus thermophilus
This part confers resistance against T7 phage upon a strain containing the Cas9 and TracrRNA parts.
This part is computationally verified to target all of 13a, K30, T3 and T7 genomes
Short segments of foreign DNA, called spacers, are incorporated into the genome between CRISPR repeats, and serve as a 'memory' of past exposures.[6] By associating with CAS9, an enzyme with both endonuclease and DNA-binding activity, CRISPR spacers are then used to recognize and silence exogenous genetic elements in a manner similar to RNAi in eukaryotic organisms. http://en.wikipedia.org/wiki/CRISPR Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]